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Bone scaffold fabrication using porogen based injection molding method and biocomposite materials
Please use this identifier to cite or link to this item:
http://hdl.handle.net/1860/1199
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| Title: | Bone scaffold fabrication using porogen based injection molding method and biocomposite materials |
| Authors: | Zhou, Jack G.
Lu, Lin
Mondrinos, Mark J.
Dembzynski, Rob
Byrapogu, Kalyan
Wootton, David
Lelkes, Peter I.
Zhou, Jack |
| Keywords: | Drop On Demand Printing (DDP)
Porogen-Based
Polycaprolactone (PCL)
Calcium Phosphate (Cap) |
| Issue Date: | 20-Jun-2006 |
| Citation: | Proceedings of the Seventh International Conference on Frontiers of Design and Manufacturing, in Guangzhou, China, June 20, 2006, 1: pp. 267-272. |
| Abstract: | Drop on demand printing (DDP) is a solid freeform fabrication (SFF) technique capable of generating
physical features required for scaffolds to be used in hard tissue repair. Here we report results toward the development
of a reproducible manufacturing process for tissue engineering scaffolds based on injectable porogens fabricated by
DDP. Thermoplastic porogens were designed using Pro/Engineer and fabricated with a commercially available DDP
machine. Scaffolds composed of either pure polycaprolactone (PCL) or homogeneous composites of PCL and calcium
phosphate (CaP, 10% or 20% w/w) were subsequently fabricated by injection molding of molten polymer-ceramic
composites. The precisely formed scaffolds were separated from the porogens in an agitated ethanol bath. Attainable
scaffold pore sizes using the porogen-based method were found to be 200 μm for pure PCL. We characterized the
compressive strength of 90:10 and 80:20 PCL-CaP composite materials (19.5+/-1.4 MPa and 24.8+/-1.3MPa
respectively) according to ASTM standards, as wells as pure PCL scaffolds (13+/-1.2 MPa) fabricated using our process.
Initial cell-biomaterial interaction studies demonstrated that our PCL and 80:20 PCL-CaP composite scaffolds
supported attachment and proliferation of human embryonic palatal mesenchymal (HEPM) cells, as evidenced by
fluorescent nuclear staining and the Alamar Blue™ assay. Scanning electron microscopy (SEM) revealed that HEPM
cells spread and demonstrated histiotypic mesenchymal morphology. |
| URI: | http://hdl.handle.net/1860/1199 |
| Appears in Collections: | Faculty Research and Publications (MEM)
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