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Bone scaffold fabrication using porogen based injection molding method and biocomposite materials
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|Title: ||Bone scaffold fabrication using porogen based injection molding method and biocomposite materials|
|Authors: ||Zhou, Jack G.|
Mondrinos, Mark J.
Lelkes, Peter I.
|Keywords: ||Drop On Demand Printing (DDP)|
Calcium Phosphate (Cap)
|Issue Date: ||20-Jun-2006 |
|Citation: ||Proceedings of the Seventh International Conference on Frontiers of Design and Manufacturing, in Guangzhou, China, June 20, 2006, 1: pp. 267-272.|
|Abstract: ||Drop on demand printing (DDP) is a solid freeform fabrication (SFF) technique capable of generating
physical features required for scaffolds to be used in hard tissue repair. Here we report results toward the development
of a reproducible manufacturing process for tissue engineering scaffolds based on injectable porogens fabricated by
DDP. Thermoplastic porogens were designed using Pro/Engineer and fabricated with a commercially available DDP
machine. Scaffolds composed of either pure polycaprolactone (PCL) or homogeneous composites of PCL and calcium
phosphate (CaP, 10% or 20% w/w) were subsequently fabricated by injection molding of molten polymer-ceramic
composites. The precisely formed scaffolds were separated from the porogens in an agitated ethanol bath. Attainable
scaffold pore sizes using the porogen-based method were found to be 200 μm for pure PCL. We characterized the
compressive strength of 90:10 and 80:20 PCL-CaP composite materials (19.5+/-1.4 MPa and 24.8+/-1.3MPa
respectively) according to ASTM standards, as wells as pure PCL scaffolds (13+/-1.2 MPa) fabricated using our process.
Initial cell-biomaterial interaction studies demonstrated that our PCL and 80:20 PCL-CaP composite scaffolds
supported attachment and proliferation of human embryonic palatal mesenchymal (HEPM) cells, as evidenced by
fluorescent nuclear staining and the Alamar Blue™ assay. Scanning electron microscopy (SEM) revealed that HEPM
cells spread and demonstrated histiotypic mesenchymal morphology.|
|Appears in Collections:||Faculty Research and Publications (MEM)|
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